NEWS: Using multiphoton microscopy to analyze aging skin
Biomedical Optics & Medical Imaging
Using multiphoton microscopy to analyze aging skin
Shulian Wu, Hui Li, Xiaoman Zhang, Zhifang Li and Shufei Xu
Applying advanced imaging and analytical techniques to measure patterns in the makeup of dermal tissue could help our understanding of how skin changes over time.
4 February 2011, SPIE Newsroom. DOI: 10.1117/2.1201012.003318
Skin undergoes structural degradation in response to both the passing of years and exposure to the sun.1–4 The degeneration of collagen, a component in the dermal extracellular matrix, is a major factor in skin alteration with aging. But the mechanism differs depending on the cause. Researchers have not yet establishing a quantitative relationship between collagen changes and different types of aging. Here, we describe work aimed at quantitatively linking collagen alteration with aging progression by multiphoton microscopy (MPM).
We have applied MPM5–7 to assess intrinsic-age-related and photo-age-related differences in skin in living organisms. This technique combines an Axiovert 200 microscope with a Zeiss LSM510 META (a 32-channel detector module) laser-scanning microscope and a Coherent Mira 900-F mode-locked femtosecond titanium:sapphire laser to detect a second-harmonic-generation (SHG) signal from collagen fibrils, which represents intrinsic phase matching in the medium. We used an excitation wavelength of 850nm and limited the average power to P≤10mW.8 As an animal model, we used 50 mice of different ages and 10 mice exhibiting photoaging and looked at the structural changes in their skin.
More: http://spie.org/x44138.xml?highlight=x2416&ArticleID=x44138
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